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Laser-Assisted Hatching (LAH)

Laser-Assisted Hatching (LAH) is a technique used during in vitro fertilization (IVF) to improve the chances of embryo implantation in the uterus.


What Is Laser-Assisted Hatching?

Every embryo is surrounded by a protective outer shell called the zona pellucida. For implantation to occur, the embryo must "hatch" out of this shell before attaching to the uterine lining.

Laser-assisted hatching uses a precise laser to create a small opening or thin a portion of the zona pellucida, helping the embryo hatch more easily.

How the Procedure Works

  1. The embryo is placed under a microscope.
  2. A laser beam (infrared, non-contact) is directed at a specific point on the zona.
  3. A small hole is made, or the zona is thinned.
  4. The embryo is returned to the incubator and transferred to the uterus shortly after.

LAH is typically performed on Day 3 or Day 5 embryos, usually just before embryo transfer.

Who Might Benefit from Laser-Assisted Hatching?

  • Advanced maternal age (usually ≥35 years)
  • Thick zona pellucida
  • Previous failed IVF cycles
  • Poor embryo quality
  • Frozen–thawed embryos (zona may harden during freezing)
  • Elevated FSH or diminished ovarian reserve

Notes & Considerations

LAH is an adjunctive lab technique and is not universally required for all IVF cycles. Its use is individualized based on clinic protocol and patient factors.

Discuss potential benefits and risks with your embryology and fertility team to determine whether LAH is appropriate for your cycle.